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Cells, 3 × 106, were treated with/without STI571 (1 µg/mL) or geldanamycin (0.1 µg/mL). Immunoprecipitated with anti‐c‐KIT (1:100) overnight at 4C°, and immunoprecipitates were protein A agarose as described in the Materials and Methods section. Anti‐heat shock protein 90 antibody (1:250) was used for Western blot analysis.
