Figure 3.

Immunoelectromicroscopic analysis. Cells were treated with/without STI571 (1 µg/mL) or geldanamycin (0.1 µg/mL). Samples were prepared as described in the Materials and Methods section. (a) Non‐treated, (b) STI571 treated, (c) geldanamycin‐treated, and (d) non‐treated, but samples were reacted with anti‐c‐KIT and 15‐nm‐gold labeled secondary antibody, and then they were reacted with anti‐heat shock protein 90 antibody and 5‐nm‐gold labeled secondary antibody.