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. 2011 Oct 10;102(11):1977–1990. doi: 10.1111/j.1349-7006.2011.02079.x

Figure 3.

Figure 3

 Biological effects of type III interferon (IFN) on apoptotic cell death. (a) Non‐small cell lung cancer (NSCLC) cell lines were cultured in medium supplemented with 50 ng/mL interleukin (IL)‐28A, 50 ng/mL IL‐29, 50 ng/mL IFN‐α, 50 ng/mL IL‐28A plus 50 ng/mL IFN‐α, 50 ng/mL IL‐29 plus 50 ng/mL IFN‐α or 20 μM cisplatin. After 24‐ or 48‐h incubation, DNA fragmentation was analyzed using a Cell Death Detection ELISA. Each value is normalized to the negative control (non‐treated) and represents the mean relative DNA fragmentation ± SD (n = 5). (b) Cells were treated with or without the indicated interferon(s) or cisplatin for 24 or 36 h. Cells were incubated with Annexin V‐PE in a buffer containing 7‐AAD and analyzed using flow cytometry. Y‐axis, cell number; X‐axis, Annexin V‐PE.