TABLE 2.

Recombinant viruses

Virus	Plaque diam (mm) with:		Heparin-Sepharose elution (mM NaCl)	ELISA reactivityb of E2 MAb:
	Agar	Agarose		R6	G5	202
Toto 1101	1.3 ± 0.4a	3.3 ± 0.7	323	+	+	+
R157H	2.5 ± 0.7	2.8 ± 0.6	289	+	+	+
N62D	2.0 ± 0.7	2.2 ± 0.6	276	−	+	+
K159E	2.8 ± 0.8	2.6 ± 0.7	261	−	+	+
K230M	2.4 ± 0.8	2.6 ± 0.7	202	+	+	+
K76N	2.3 ± 0.9	2.5 ± 0.7	142	+	+	+
K76T	2.3 ± 0.8	2.6 ± 0.8	97	+	+	+
K76E	3.1 ± 0.8	2.5 ± 0.8	66	+	+	+

^aThe plaque size (mean ± standard deviation) of Toto 1101 under agar was significantly smaller (P, <0.05) than those of all other viruses under agar and was also significantly smaller than that of Toto 1101 under agarose (Kruskal-Wallis analysis of variance ranks followed by Dunn's multiple-comparison test). 

^b+, reactivity; −, no reactivity.