Table 2.

Positions and sequences of the primers used for polymerase chain reaction (PCR) amplification and sequencing

Position †	Nucleotide sequence (5′–3′)	Polarity
PCR primer
p1 : 1821–1841	TTTTTCACCTCTGCCTAATCA	Sense
p2 : 1825–1806	AAAAAGTTGCATGGTGCTGG	Antisense
p3 : 1922–1940	GAATTTGGAGCTTCTGTGG	Sense
p4 : 1788–1771	GCCTACAGCCTCCTAGTA	Antisense
p5 : 1604–1623	TCGCATGGAGACCACCGTGA	Sense
p6 : 2076–60	ATAGCTTGCCTGAGTGC	Antisense
p7 : 1653–1672	CATAAGAGGACTCTTGGACT	Sense
p8 : 1974–1957	GGAAAGAAGTCAGAAGGC	Antisense
Sequencing primer
242–258	CAGAGTCTAGACTCGTGG	Sense
687–668	GGCACTAGTAAACTGAGCCA	Antisense
456–475	AAGGTATGTTGCCCGTTTGT	Sense
771–752	TACAGACTTGGCCCCCAATA	Antisense
668–687	TGGCTCAGTTTACTAGTGCC	Sense
1103–1086	GGCGAGAAAGTGAAAGCC	Antisense
1054–1073	ATGCCTTTATATGCATGTAT	Sense
1436–1418	GACGGGACGTAGACAAAGG	Antisense
1267–1285	CATACTGCGGAACTCCTAGC	Sense
2470–2453	TTATGAGTCCAAGGGATA	Antisense
2301–2320	CACCAAATGCCCCTATCTTA	Sense
2656–2639	GGATAGAACCTAGCAGGC	Antisense
2637–2656	ATGCCTGCTAGGTTCTATCC	Sense
3155–3138	CTTCCTGACTGCCGATTG	Antisense
3082–3099	CCTCAGGCTCAGGGCATA	Sense
475–456	ACAAACGGGCAACATACCTT	Antisense

^† Nucleotide position based on the sequence of hepatitis B virus (HBV) genotype C (accession no. X01587). Primers p3 to p8 were also used for sequencing.