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Effect of 58‐kDa microspherule protein (MSP58) siRNA on cell cycle effector levels by western blotting. Cyclin D1, cyclin‐dependent kinase (CDK) 4, phosphorylation Retino blastoma (p‐Rb), retinoblastoma (Rb), and p21 proteins were evaluated in HT‐29 cells transfected with the indicated plasmids. β‐Actin was used as an internal control.
