Skip to main content
. 2009 Oct 22;101(2):494–500. doi: 10.1111/j.1349-7006.2009.01412.x

Figure 3.

Figure 3

 Rapamycin inhibits αv integrin signaling of B16 cells. (a,b) Rapamycin down‐regulated the expression of αv integrin in B16 cells. B16 cells were treated with rapamycin in vitro. Twenty‐four hours later, the cells were harvested for the detection of αv and β3 expression by real‐time RT‐PCR (a); or 24 h later, the cells were used for Western blot analysis (b). (c) Rapamycin inhibited the activation of focal adhesion kinase (FAK). B16 cells were treated with rapamycin for 24 h. The phosphorylated FAK and total FAK were analyzed by Western blotting. (d) Assay of cdc2 expression. The rapamycin‐treated cells were used for total RNA isolation and cell lysate preparation. The expression of cdc2 was analyzed by real‐time RT‐PCR (bottom) and Western blotting (up). (e,f) Assay of matrix metalloproteinase (MMP)‐2 and MMP‐9 production. B16 cells were cultured in the presence or absence of rapamycin for 24 h. MMP‐2 and MMP‐9 in supernatants were detected by zymography assay (e). The relative mRNA levels of MMP‐2 and MMP‐9 were detected by real‐time RT‐PCR (f).