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Cell cycle analysis. 3T3‐fibrobrast cells were cultured at 37°C for 24 h with 20 μm 20‐O‐ingenolEZ. Mouse breast cancer (MMT) cells were cultured at 37°C for 12, 24, and 48 h with 20‐O‐ingenolEZ at 10 μg/mL. The cells stained with propidium iodide were subjected to flow‐cytometric analysis (a). (b) Shows the results of (a) with % the cell distributions in each G1, S, and G2/M phase of the cell cycle in untreated cells and at 12, 24, and 48 h after 20‐O‐ingenolEZ treatment.
