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Figure 3.

Figure 3

Expression of HSP105 protein and infiltration of CD4+ T cells and CD8+ T cells in the HSP105 DNA vaccine‐injected sites. To observe HSP105 expression and infiltrating cells in muscles injected with the HSP105 DNA vaccine, we carried out intramuscular immunizations with pCAGGS‐DNA into the right anterior tibialis muscle, and with pCAGGS‐HSP105 DNA into the left anterior tibialis muscle in four mice. After 48 h, we killed the mice and studied their muscle tissue by using HE staining and histological analysis, and immunohistochemical analysis of HSP105, CD4, and CD8. Representative results are shown. Objective magnification was 400×.