Figure 3.

(a) Morphological changes in TE3 and TE7 cells 72 h after a 24‐h treatment with oxaliplatin (OHP; 2.5 µM for both) or cisplatin (CDDP; 2.5 µM for TE3, 3 µM for TE7). Apoptotic cells were observed frequently in treated TE3 cells, whereas multiple or enlarged nuclei were observed in TE7 cells after treatment. Cells with fragmented nuclei or condensed DNA are labeled with dotted arrows and giant multinucleated cells are labeled with bold arrows. Original magnification ×400. (b) Percentage of cells with two or more nuclei (undergoing mitotic catastrophe). TE3 and TE7 cells were treated as in (a), fixed at the time indicated, stained, and observed using light microscopy. A gradual increase in the percentage of cells undergoing mitotic catastrophe was observed in TE7 cells after OHP treatment. Changes in treated groups were compared with control cells. The statistical significance (P‐value) is indicated.