Figure 8.

Survivin regulation. (a) Effects of a proteasome inhibitor (lactacystin) on survivin expression following oxaliplatin (OHP) treatment. (b) Total Stat3 and phosphorylated (Tyr705) Stat3 protein expression. (c) Sp1 expression at 48 h after OHP (2.5 µM) treatment. Actin was used as a loading control. (d) Effect of Stat3 inhibitor peptide and (e) Sp1 inhibitor (mithramycin A) on survivin promoter activity at 24 h after transfection in two esophageal (TE7 and TE3) and two gastric (MKN45 and MKN28) cancer cell lines. Luciferase activity was normalized against Renilla luciferase activity, and the control (without treatment) was adjusted to 100%. The statistical significance (P‐value) is indicated.