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Detection of methylated and unmethylated breast cancer gene 1 (BRCA1) by methylation‐specific PCR. TE buffer was used as reagent control. Genomic DNA of human breast cancer cell line MCF7 was used as the unmethylated BRCA1 negative control (NC). The M.Sss I‐modified genomic DNA of blood from healthy persons was used as the methylated BRCA1 positive control (PC). Methylation‐specific PCR products (M, methylated, 75 bp; U, unmethylated, 86 bp) were run on an 8% PAGE gel.
