Skip to main content
. 2008 Nov 27;100(1):117–125. doi: 10.1111/j.1349-7006.2008.01017.x

Figure 1.

Figure 1

Basal levels of extracellular signal‐regulated kinase/mitogen‐activated protein kinase (ERK‐MAPK) phosphorylation in human gastric epithelial cells and constitutively active RAF (caRAF) transfected cells. (a) The expression of MEK/ERK phosphorylation of cancer cell AGS and epithelial cell GES‐1 after stimulation with serum for 24 h and western blot analysis with antibodies specific for phospho‐MEK1/2 and phospho‐ERK1/2. To control for loading, the membrane was stripped and reblotted with antitotal‐MEK1/2 or antitotal‐ERK1/2 serum. (b) The expression of phospho‐B‐RAF increased in caRAF‐AGS and caRAF‐GES‐1 compared with vehicle control, β‐actin served as a control for loading. (c) The expression of phosphorylated MEK and phosphorylated ERK in caRAF expressing cells.