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. 2008 Nov 27;100(1):117–125. doi: 10.1111/j.1349-7006.2008.01017.x

Figure 2.

Figure 2

Effects of constitutively active RAF (caRAF) or PD98059 on cell proliferation. Cell proliferation was measured by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenytetra‐zolium) (MTT) assay. caRAF‐GES‐1 and caRAF‐AGS were seeded in 12‐well plates and cultured in complete media. (a) Relative growth rates of these cells were compared for 7 days. (b) The vitality of caRAF‐GES‐1 and caRAF‐AGS, as well as the OD value of both cells treated with PD98059 or dimethylsulfoxide (DMSO) for 48 h. The effective inhibited concentrations of PD98059 for caRAF‐GES‐1 was 25 µM and caRAF‐AGS was 50 µM. (c) Quantification of transformed caRAF‐GES‐1 cells as foci after 14 days in culture from triplicate plates, the data from soft agar assay.