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. 2008 Nov 27;100(1):117–125. doi: 10.1111/j.1349-7006.2008.01017.x

Figure 6.

Figure 6

The expressions of Dnmt1 and Dnmt3b in mRNA or protein levels were upregulated in constitutively active RAF (caRAF)‐GES‐1. PD98059 reversed the upregulation of Dnmt1. Total RNA and protein from GES‐1 expressing caRAF or vector only, along with caRAF‐GES‐1 cells treated with 25 µM PD98059. (a) The graphical representation of the Dnmt1, Dnmt3a and Dnmt3b mRNA, data are mean ± standard deviation of triplicates of a representative experiment. Similar results were obtained in two experiments. (b) Cell lysates were used for analyzed by western blot with antibody specific for Dnmt1, Dnmt3a and Dnmt3b, β‐actin serving as a loading control. (c) caRAF can increase the activity of DNA methyltransferase in GES‐1 (216 ± 13%) and AGS (193 ± 32%) cell lines compared with each control. DMSO, dimethylsulfoxide.