Figure 4.

(A) Time‐dependent induction of Ets‐1 and vascular endothelial growth factor (VEGF) expression by hypoxia. Renal cell carcinoma cell lines were incubated under hypoxic conditions (1% O₂). Total RNA was isolated at the time points indicated after hypoxic stimulation, and reverse transcription–polymerase chain reaction (RT‐PCR) was carried out. Note that hypoxic treatment increased Ets‐1 and VEGF mRNA expression in 786‐O, 769‐P and ACHN cell lines, and induced Ets‐1 expression in A498 cells. (B) Effect of neutralizing anti‐VEGF antibody and recombinant human VEGF₁₆₅ protein on Ets‐1 expression. 786‐O and ACHN cells were cultured for 2 days with or without anti‐VEGF antibody (2.5 µg/mL) and/or recombinant human VEGF₁₆₅ protein (50 ng/mL), and the Ets‐1 expression was analyzed by RT‐PCR.