Figure 4.
Combination of programmed cell death 4 (PDCD4) with cisplatin inhibited tumor growth in nude mice. SKOV3 ovarian cancer cells were inoculated s.c. When tumors reached approximately 100 mm3, mice were randomly divided into six groups and treated by intratumoral injection with normal saline (NS), empty vector (MOCK), PDCD4 plasmid, cisplatin, MOCK + cisplatin, or PDCD4 + cisplatin. (a) Tumors were measured at different days after treatment and growth curves were drawn. Tumor volume of the PDCD4 + cisplatin group was significantly smaller than those of PDCD4, cisplatin, and MOCK + cisplatin groups (*P < 0.05). (b) Transplanted tumors were excised after different treatments. (c) Tumor weight of six different groups was measured. The tumor weight of the combination treatment group was significantly reduced compared with other groups (*P < 0.05; **P < 0.01). The results are expressed as the mean of each treatment group ± SEM. (d) Expression of PDCD4 in tumor samples detected by immunohistochemistry. Images shown are representative of each group. Magnification, ×400.