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Detection of surface‐expressed filamin A (FLNa) in LAN‐1 cells. Subconfluent cells were surface‐biotinylated, lyzed, and immunoprecipitated with the anti‐FLNa antibody MAB1678. Cell lysates (right panel) or precipitated proteins (left panel) were resolved by 7.5% SDS‐PAGE under reducing conditions, electroblotted onto a PVDF membrane, and probed with the MAB1678 antibody or streptavidin.
