Table 2.
Sequences of primers for PCR amplification
| 2nd Mix | TRBV gene names† | Forward primer sequence (5′–3′) | |
|---|---|---|---|
| PCR forward primers (TCR‐BV gene family ‐specific primers) | S1 | TRBV9 | ACAGCAAGTGAC<TAG>CTGAGATGCTC |
| TRBV5 | |||
| TRBV25 | GATCACTCTGGAATGTTCTCAAACC | ||
| TRBV10 | CCAAGACACAAGGTCACAGAGACA | ||
| S2 | TRBV20 | GAGTGCCGTTCCCTGGACTTTCAG | |
| TRBV28 | GTAACCCAGAGCTCGAGATATCTA | ||
| TRBV2 | GGTCACACAGATGGGACAGGAAGT | ||
| S3 | TRBV29 | TCCAGTGTCAAGTCGATAGCCAAGTC | |
| TRBV7 | ATGTAACT<CT>TCAGGTGTGATCCAA | ||
| TRBV27 | GTGACCCAGAACCCAAGATACCTC | ||
| S4 | TRBV7 | GTGTGATCCAATTTCAGGTCATAC | |
| TRBV12 | GGTGACAGAGATGGGACAAGAAGT | ||
| TRBV11 | CAGTCTCCCAGATATAAGATTATAGAG | ||
| S5 | TRBV19 | CACTCAGTCCCCAAAGTACCTGTT | |
| TRBV30 | GTCAGATCTCAGACTATTCATCAATGG | ||
| TRBV4 | TACGCAGACACCAA<GA>ACACCTGGTCA | ||
| TRBV3 | CCCAGACTCCAAAATACCTGGTCA | ||
| TRBV18 | TGCAGAACCCAAGACACCTGGTCA | ||
| S6 | TRBV21 | AAGGTCACCCAGAGACCTAGACTT | |
| TRBV14 | ATAGAAGCTGGAGTTACTCAGTTC | ||
| TRBV23 | ACAAAGATGGATTGTACCCCCGAA | ||
| S7 | TRBV6 | GTGTCACTCAGACCCCAAAATTCC | |
| TRBV24 | GTTACCCAGACCCCAAGGAATAGG | ||
| S8 | TRBV13 | CTGATCAAAGAAAAGAGGGAAACAGCC | |
| TRBV15 | CAAGATACCAGGTTACCCAGTTTG | ||
| Reverse primer sequence (5′–3′) | 1st‐PCR (3′BC) | GCTTCTGATGGCTCAAACACAGC | |
| Screening and specifying‐PCR (5′BC) | GGAACACGTTTTTCAGGTCCT | ||
†TCR‐BV gene names are in accordance with IMGT unique gene nomenclatures. < >, mixture of nucleotides at this position.