Table 2.
2nd Mix | TRBV gene names† | Forward primer sequence (5′–3′) | |
---|---|---|---|
PCR forward primers (TCR‐BV gene family ‐specific primers) | S1 | TRBV9 | ACAGCAAGTGAC<TAG>CTGAGATGCTC |
TRBV5 | |||
TRBV25 | GATCACTCTGGAATGTTCTCAAACC | ||
TRBV10 | CCAAGACACAAGGTCACAGAGACA | ||
S2 | TRBV20 | GAGTGCCGTTCCCTGGACTTTCAG | |
TRBV28 | GTAACCCAGAGCTCGAGATATCTA | ||
TRBV2 | GGTCACACAGATGGGACAGGAAGT | ||
S3 | TRBV29 | TCCAGTGTCAAGTCGATAGCCAAGTC | |
TRBV7 | ATGTAACT<CT>TCAGGTGTGATCCAA | ||
TRBV27 | GTGACCCAGAACCCAAGATACCTC | ||
S4 | TRBV7 | GTGTGATCCAATTTCAGGTCATAC | |
TRBV12 | GGTGACAGAGATGGGACAAGAAGT | ||
TRBV11 | CAGTCTCCCAGATATAAGATTATAGAG | ||
S5 | TRBV19 | CACTCAGTCCCCAAAGTACCTGTT | |
TRBV30 | GTCAGATCTCAGACTATTCATCAATGG | ||
TRBV4 | TACGCAGACACCAA<GA>ACACCTGGTCA | ||
TRBV3 | CCCAGACTCCAAAATACCTGGTCA | ||
TRBV18 | TGCAGAACCCAAGACACCTGGTCA | ||
S6 | TRBV21 | AAGGTCACCCAGAGACCTAGACTT | |
TRBV14 | ATAGAAGCTGGAGTTACTCAGTTC | ||
TRBV23 | ACAAAGATGGATTGTACCCCCGAA | ||
S7 | TRBV6 | GTGTCACTCAGACCCCAAAATTCC | |
TRBV24 | GTTACCCAGACCCCAAGGAATAGG | ||
S8 | TRBV13 | CTGATCAAAGAAAAGAGGGAAACAGCC | |
TRBV15 | CAAGATACCAGGTTACCCAGTTTG | ||
Reverse primer sequence (5′–3′) | 1st‐PCR (3′BC) | GCTTCTGATGGCTCAAACACAGC | |
Screening and specifying‐PCR (5′BC) | GGAACACGTTTTTCAGGTCCT |
†TCR‐BV gene names are in accordance with IMGT unique gene nomenclatures. < >, mixture of nucleotides at this position.