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. 2009 Jun 26;100(10):1848–1855. doi: 10.1111/j.1349-7006.2009.01262.x

Figure 1.

Figure 1

Sonic Hedgehog (SHH) pathway components were highly expressed in human primary neuroblastomas (NBs) and NB cell lines. The expression of SHH, Patched 1 (PTCH1), Smoothened (SMO), and GLI2 in 40 human NB specimens was characterized by immunohistochemistry. Protein expression of SMO, GLI2, and SHH in human NB cells was characterized by immunoblotting. Data are representative images from groups of tissues or immunoblots. The expression of α‐tubulin was used as the control. (a) Immunohistochemical (IHC) staining for SHH, PTCH1, SMO, and GLI2 in NB specimens. Positive cells stained brown. Scale bars, 100 µm. (b) Immunoblot analysis of SMO in human NB cell lines. (c) Immunoblot analysis of SHH and GLI2 expression in NB cell lines. SHH undergoes autocatalytic processing to generate SHH‐N, a 19‐kDa protein with SHH signaling activity.