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. 2009 Jun 26;100(10):1848–1855. doi: 10.1111/j.1349-7006.2009.01262.x

Figure 3.

Figure 3

Sonic Hedgehog (SHH) pathway activation is required for the tumorigenicity of neuroblastoma (NB) cells. BE(2)‐C and SK‐N‐DZ cells were treated with 20 µM cyclopamine and/or 3 µg/mL SHH or the vehicle in a soft agar culture system for 2–3 weeks, and the formed colonies were stained with MTT and imaged in order to count colonies. Data represent the average ± SD of at least three independent experiments. Statistical analysis was performed using the two‐tailed Student's t‐test. V, treated with ethanol alone; C20, 20 µmol/L Cyclopamine; S3, 3 µg/mL SHH. Scale bar, 2 mm.