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. 2009 Jun 26;100(10):1848–1855. doi: 10.1111/j.1349-7006.2009.01262.x

Figure 5.

Figure 5

Sonic Hedgehog (SHH) pathway activation affects apoptosis of neuroblastoma (NB) cells. Three types of NB cell lines were treated with 20 µM cyclopamine and/or 3 µg/mL SHH or the vehicle for 48 h and apoptosis was analyzed by FACS. The Annexin V+/PI– cells were considered to be in the early stage of apoptosis, the Annexin V+/PI+ cells were in the late stage of apoptosis, Annexin V–/PI+ cells were necrotic, and Annexin V–/PI– cells were healthy cells. Data are expressed as the mean percent ± SD of each group of cells from three independent experiments. V, treated with ethanol alone; C20, 20 µmol/L Cyclopamine; S3, 3 µg/mL SHH.