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. 2009 Mar 23;100(6):1069–1074. doi: 10.1111/j.1349-7006.2009.01136.x

Figure 2.

Figure 2

Alpha subunit of stimulatory heterotrimeric GTP‐binding protein (Gαs) augments cisplatin‐induced apoptosis by regulating the expression of B‐cell leukemia/lymphoma‐2 genes (Bcl‐2) family proteins in A549 cells. (a,b) Effects of Gαs on the expression level of Bcl‐2 family proteins in cisplatin‐treated A549 cells. (a) A549 cells were treated with 40 µM cisplatin for 24 h, and expression of Bcl‐2 family proteins was analyzed by western blotting. (b) The densitometric results are presented as histograms. (c) Effects of forskolin on the expression of Bcl‐2 family proteins and cisplatin‐induced apoptosis. A549 cell were pretreated with 7.5 µM forskolin and 100 µM isobutylmethylxanthine in dimethyl sulfoxide for 1 h and then with 40 µM cisplatin for 24 h before analysis of the expression of Bcl‐2 family proteins by western blotting. (d) Augmentation of cisplatin‐induced apoptosis of H1299 lung cancer cell by Gαs. GαsQL was also stably expressed in H1299 human lung cancer cells, which were treated with 40 µM cisplatin for 24 h, and the Bcl‐2 family proteins, and cleavage of caspase‐3 and poly (ADP‐ribose) polymerase (PARP) were analyzed by western blotting. Bak, Bcl‐2 homologous antagonist killer protein; Bax, Bcl‐2 associated X protein; CREB, cyclic AMP response element binding protein.