Figure 2.

Cyclins and active caspase‐3 double staining analysis of leukemia cells treated with anti‐Fas antibody. (a) PEER and (b) Jurkat cells were pretreated with anti‐Fas antibody (50 ng/mL) for 0, 2, 4 and 6 h. For intracellular staining, cells were first fixed and permeabilized, then stained with fluorescein isothiocyanate‐conjugated antiactive caspase‐3 antibody and phycoerythrin (PE)‐conjugated anticyclin A/B1/E, then analyzed by flow cytometry. Similar results were obtained in three repeated experiments.