Figure 5.

Cyclins and active caspase‐8 double staining analysis of leukemia cells treated with anti‐Fas antibody. (a) PEER and (b) Jurkat cells were incubated with anti‐Fas (50 ng/mL) antibody for 0, 2, 4 and 6 h. Before intracellular staining, cells were preincubated with FAM‐peptide‐FMK (peptide caspase‐8), then fixed and permeabilized and stained with phycoerythrin (PE)‐conjugated cyclin A/B1 and analyzed by flow cytometry. For cyclin E staining, cells were additionally stained with PE‐conjugated secondary antibody. Similar results were obtained in three repeated experiments.