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. 2009 Jun 17;100(10):1902–1907. doi: 10.1111/j.1349-7006.2009.01245.x

Figure 3.

Figure 3

Lower expression of Coxsackie virus and adenovirus receptor (CAR) and much higher expression of integrin β3 in radioresistant cells were associated with decreased adenovirus gene expression (A) (a) CAR and (b) integrin β3 mRNA expression in cells. CAR and integrin β3 mRNA were quantified by real‐time RT‐PCR from total RNA from radiosensitive cells (CFPAC‐1 parent cells) or radioresistant cells (R1, R2) and defined as the ratio relative to radiosensitive cells. (B and C) Inhibition of integrin β3 mRNA expression by siRNA‐recovered NK4 expression in radioresistant cells. (B) Integrin β3 mRNA was quantified by real‐time RT‐PCR from total cellular RNA from integrin β3‐specific siRNA‐transfected cells or control siRNA‐transfected cells at 24, 48, and 72 h after transfection and was expressed as fold‐decrease compared with control siRNA cells. Each value represents the mean ± SD of triplicate measurements. (C) Radioresistant cells were transfected with integrin β3‐specific siRNA or control siRNA and infected with Ad‐NK4 at 10 multiplicities of infection at 48 h after transfection. NK4 levels in the culture media were measured by enzyme‐linked immunosorbent assay and expressed as fold‐increase compared with control siRNA cells. Each value represents the mean ± SD of three independent samples. *P < 0.05.