Table 2.
TLR expression profiles in human DC subsets
| Freshly isolated | In vitro‐differentiated | ||||
|---|---|---|---|---|---|
| Monocytes | mDC* | pDC** | DCs | Macrophages | |
| TLR1 | ++ | + | − | + | ++ |
| TLR2 | ++ | ++ | − | ++ | ++ |
| TLR3 | − | ++ | − | ++ | + |
| TLR4 | ++ | + | − | + | + |
| TLR6 | ++ | + | − | + | + |
| TLR7 | − | − | + | − | − |
| TLR8 | + | + | − | + | + |
| TLR9 | − | − | + | − | − |
Positive and negative symbols denote the results of the flow cytometry (FACS) analyses using monoclonal antibodies, except TLR7, TLF8, and TLR9. Results were determined by reverse transcription–polymerase chain reaction. TLR3, TLR7, TLR8, and TLR9 reside in the endosome to recognize nucleotide derivatives. (*) PDCA1+ cells; (**) PDCA4+ cells. PDCA, plasmacytoid dendritic cell antigen; DC, dendritic cell; mDC, myeloid dendritic cells; pDC, plasmacytoid dendritic cells; TLR, Toll‐like receptor.