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. 2008 Dec 18;100(3):481–489. doi: 10.1111/j.1349-7006.2008.01054.x

Figure 5.

Figure 5

Discrepancy between the presence of Tax‐specific cytotoxic T‐lymphocytes (CTLs) and interferon (IFN)‐γ production in peripheral blood mononuclear cells (PBMCs) from asymptomatic human T‐cell leukemia virus type‐1 (HTLV‐1) carriers (ACs). (a) Uncultured PBMCs from indicated subjects were stained with phycoerythrin (PE)‐Cy5‐conjugated CD8 monoclonal antibody (mAb) together with PE‐conjugated HLA‐A*1101/Tax88‐96 tetramers for subject #238, and HLA‐A*2402/Tax301‐309 tetramers for subjects #211 and #244. The percentages of tetramer+ cells among CD8+ cells are indicated, as analyzed by flow cytometry. (b) CCR4+ cell‐depleted PBMCs from the same subjects were incubated with control dimethylsulfoxide (DMSO) (open bars) or 10 µM of Tax peptides (closed bars) for 4 days, and the amounts of IFN‐γ in the supernatants were measured by enzyme‐linked immunoabsorbent assay. Tax peptides used were Tax 88‐96 for subject #238 and Tax 301‐309 for subjects #211 and #244. The values represent folds of IFN‐γ production against control DMSO, and indicated as the mean ± SD of duplicate samples.