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. 2010 Jan 28;101(5):1117–1124. doi: 10.1111/j.1349-7006.2010.01515.x

Figure 2.

Figure 2

 Effect of brain‐derived neurotrophic factor (BDNF) on vascular endothelial growth factor (VEGF) secretion in bone marrow stromal cells. (a) Western blot analysis demonstrating tropomyosin receptor kinase B (TrkB) expression by human bone marrow stromal cells (BMSCs). (b) VEGF concentrations were determined in supernatant of BMSC cultures exposed to various concentrations of BDNF (0–200 ng/mL) for 48 h. (c) VEGF concentrations were determined in supernatants of BMSCs exposed to 100 ng/mL of BDNF at various time points (•), unstimulated controls (△). (d) As a control experiment, stimulation with 100 ng/mL BDNF was also performed with or without K252α pretreatment (200, 500 nm; 30 min) in BMSC cultures. VEGF in supernatants at various time points (0, 12, 24, 48 h) was measured by ELISA. (e) Enhanced VEGF secretion of BMSCs in Transwell cocultures with RPMI‐8226 cells (P < 0.001 vs BMSCs). The addition of K252α results in a significant reduction of VEGF secretion (P < 0.001). Down‐regulation of BDNF expression inhibits VEGF secretion in Transwell cocultures of RPMI‐8226 and BMSCs (P < 0.001).