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. 2010 May 22;101(9):1990–1996. doi: 10.1111/j.1349-7006.2010.01626.x

Figure 1.

Figure 1

 Clast1/LR8 expression is downregulated as a result of oncogenic Ras activation. (a) Total RNA extracts prepared from parental NIH3T3 cells, NIH3T3/vector cells, and H‐RasV12 NIH3T3 cells were analyzed by semiquantitative RT‐PCR using mouse Clast1/LR8‐specific primers. (b) WI38 cells were transiently transfected with an H‐RasV12 expression vector or control vector. Total RNA was isolated and human Clast1/LR8 mRNA expression was then examined by RT‐PCR using human Clast1/LR8‐specific primers. (c) Ras activity (upper panel) and Clast1/LR8 mRNA expression (determined by RT‐PCR) (lower panel) in four lung cancer cell lines. (d) Clast1/LR8 expression in H460 and A549 cells transfected with control siRNA, K‐Ras siRNA#1, or K‐Ras siRNA #2.