Figure 4.

 Clast1/LR8 expression inhibits an H‐RasV12‐mediated increase in colony formation in soft agar. (a) The indicated stable transfectants of NIH3T3 and H‐RasV12 NIH3T3 cells (2 × 10⁴) were plated in medium containing 0.3% agar and supplemented with 10% FBS on a 0.5% agar base. Representative images of colony formation were taken after 20 days of growth. (b) Percentages of cells present in colonies >50 μm in diameter. Values represent the mean ± SD of three separate experiments. **P < 0.01.