Figure 4.
Comparison among spliceostatin A (SSA), FR901464 and ACR in their ability to suppress in vivo blood vessel formation induced by HeLa cells in chicken chorioallantoic membrane (CAM) tissues (gelatin sponge‐CAM assay). (a) HeLa cells were delivered at 3 × 105 cells per embryo onto the top of the CAM at day 8 using a gelatin sponge implant, supplemented with or without SSA (2 nM), FR901464 (0.2, 2 and 20 nM) and acyclic retinoid (ACR) (0.05, 0.5 and 5 μM) as a positive control. At day 12, newly formed microvessels sprouting towards the implant were observed under the microscope. Representative results are shown. g. s., represents the area of gelatin sponge; c. a., represents the count area meaning the area in which we counted microvessels. Black scale bars represent 500 μm. (b) The numbers of the microvessels inside the same area (surrounded by squares) were counted. Each datum represents the average ±SD (n = 6–7). **P < 0.01; *P < 0.05. NS, not significant.