Figure 3.

Methylation analysis of the Wnt inhibitory factor‐1 (WIF‐1) promoter in Barrett's esophagus (BE) tissue samples. (a) WIF‐1 promoter methylation by methylation‐sensitive single‐strand conformation analysis in four BE patients without esophageal adenocarcinoma (EAC) and in four BE patients with EAC. Square brackets indicate the migration of either the umethylated DNA (unmethyl. CpGs) or the methylated DNA (methyl. CpGs). + and – indicate the methylation status of each sample. (b) Graphical representation of the methylated and unmethylated samples of BE without and BE with EAC.