Table 2.
List of proteins identified as being differentially expressed
Spot no. | Protein AC † | Protein name | M r (Da) ‡ | pI § | MASCOT score ¶ | Sequence coverage (%) ‡‡ |
---|---|---|---|---|---|---|
Summary of identified proteins upregulated in the metastatic CL1‐5 cell line | ||||||
U1 | P11021 | 78 kDa glucose‐regulated protein (precursor) | 72333 | 5.07 | 178 | 25 |
U2 | Q9Y224 | Protein C14orf166 | 28068 | 6.19 | 129 | 43 |
U3 | P08670 | Vimentin | 53520 | 5.06 | 172 | 29 |
U4 | P11142 | Heat shock 70 kDa protein 8 | 70898 | 5.37 | 190 | 33 |
U5 | P05783 | Cytokeratin 18 | 47927 | 5.34 | 89 | 33 |
U6 | P05787 | Cytokeratin 8 | 53543 | 5.37 | 114 | 38 |
U7 | P06733 | Alpha enolase | 47038 | 6.99 | 145 | 33 |
U8 | O76003 | Thioredoxin‐like protein 2 | 37432 | 5.31 | 168 | 42 |
U9 | P52565 | Rho GDP‐dissociation inhibitor 1 (GDIR) | 23207 | 5.02 | 86 | 31 |
U10 | P60174 | Triosephosphate isomerase | 26538 | 6.51 | 95 | 38 |
U11 | P09429 | High mobility group protein 1 | 24763 | 5.62 | 94 | 61 |
U12 | P16949 | Stathmin | 17171 | 5.87 | 129 | 51 |
U13 | O14737 | Programmed cell death protein 5 | 14154 | 5.78 | 82 | 38 |
U14 | Q9NRX4 | 14 kDa phosphohistidine phosphatase | 13833 | 5.99 | 97 | 36 |
U15 | P09382 | Galectin‐1 | 14585 | 5.34 | 106 | 41 |
U16 | P31949 | S100A11 | 11740 | 7.18 | 110 | 45 |
Summary of identified proteins downregulated in the metastatic CL1‐5 cell line | ||||||
D1 | P04083 | Annexin A1 | 38583 | 7.02 | 133 | 52 |
D2 | Q99439 | Calponin‐2 | 33566 | 6.92 | 76 | 22 |
D3 | O00625 | Pirin | 32113 | 6.42 | 93 | 25 |
D4 | Q06830 | Peroxiredoxin‐1 | 22110 | 8.27 | 67 | 35 |
D5 | P30043 | Flavin reductase | 21988 | 7.31 | 86 | 35 |
D6 | P30086 | Phosphatidylethanolamine‐binding protein 1(RKIP) | 20925 | 7.42 | 153 | 54 |
D7 | P22392 | Nucleoside diphosaphate kinase B (nm23‐H2) | 17298 | 8.52 | 73 | 33 |
D8 | P49903 | Selenide, water dikinase 1 | 42911 | 5.64 | 126 | 33 |
D9 | Q14847 | LIM and SH3 domain protein 1 (LASP1) | 29717 | 6.61 | 83 | 20 |
D10 | P09936 | PGP9.5 (UCHL1) | 24824 | 5.33 | 94 | 51 |
D11 | P62993 | Growth factor receptor‐bound protein 2 | 25206 | 6.31 | 71 | 29 |
D12 | P04792 | HSP 27 | 22783 | 5.98 | 72 | 25 |
D13 | P32119 | Peroxiredoxin‐2 | 21892 | 5.67 | 90 | 34 |
D14 | O75347 | Tubulin‐specific chaperone A | 12724 | 5.07 | 99 | 37 |
D15 | Q9UHV9 | Prefoldin subunit 2 | 16648 | 6.20 | 123 | 46 |
D16 | Q99471 | Prefoldin subunit 5 | 17328 | 5.93 | 93 | 49 |
D17 | P07108 | Acyl‐CoA‐binding protein | 9913 | 6.55 | 89 | 43 |
SWISS‐PROT accession number of each protein.
‡ Theoretical molecular weight of the matching protein in Da.
§ Theoretical isoelectric point of the matching protein.
¶ MASCOT score for SWISS‐PROT database search based on PMF.
‡‡ Percentage of identified sequence compared to the complete sequence of the known protein.