Figure 4.

Evaluation of the test proteins for association with MT1‐MMP. (a) Schematic illustration of the test proteins tagged with FLAG at the C‐terminus and c‐Myc‐tagged MT1‐MMP at the N‐terminus (MT1‐WT). FLAG, FLAG Epitope; c‐Myc, c‐Myc epitope; CAT, catalytic domain; H, hinge domain; HPX, hemopexin‐like domain; TM, transmembrane domain. (b) HT1080 cells were transiently cotransfected with the expression plasmids for the indicated test proteins and MT1‐WT. After cell lysis, an immunoprecipitation assay was performed by using anti‐FLAG antibody‐conjugated agarose. The precipitates were analyzed by Western blot analysis using indicated antibodies. Arrowheads indicate pro‐ and processed MT1‐WT.