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. 2009 Mar 27;100(7):1284–1290. doi: 10.1111/j.1349-7006.2009.01173.x

Figure 5.

Figure 5

Immuno‐localization assay of the test proteins. HT1080 cells were transfected with plasmids for c‐Myc tagged MT1‐MMP (MT1‐WT) and test FLAG‐tagged proteins. Cells were then stained with antic‐Myc antibody (orange) and anti‐FLAG polyclonal antibody (green) after permeabilization, and analyzed by confocal laser microscopy. (a) 5T4 antigen colocalized with MT1‐MMP at the ruffling membrane area, (b) CPD was detected mainly in the intracellular vesicles but not at the ruffling membrane, and (c) CLA‐1 failed to precipitate MT1‐MMP, and was detected in the cytoplasmic vesicles. Arrows show colocalized MT1‐MMP and FLAG‐tagged test proteins.