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. 2005 Jul 29;96(7):425–433. doi: 10.1111/j.1349-7006.2005.00063.x

Figure 7.

Figure 7

Effect of inhibitors of STAT3 and AKT signaling on levels of PSA in LNCaP cells. (a) Western blot analysis. LNCaP cells were cultured with either control diluent (0.25% DMSO), JAK2 inhibitor AG490 (5 × 10−5 M) or PI3‐K inhibitor LY294002 (5 × 10−5 M). After 24 h, cells were harvested and proteins were extracted and subjected to western blot analysis. The polyvinylidene fluoride membrane was sequentially probed with anti‐PSA, ‐p‐AKT (Ser473), ‐AKT, and ‐β‐actin antibodies. (b) STAT3 ELISA: LNCaP cells were cultured with either control diluent (0.25% DMSO), JAK2 inhibitor AG490 (5 × 10−5 M) or PI3‐K inhibitor LY294002 (5 × 10−5 M) for 24 h. Nuclear protein was extracted and subjected to ELISA for measurement of STAT3 DNA binding activity. Results represent the mean ± SD of two experiments carried out in duplicate.