Skip to main content
. 2009 Jun 17;100(10):1809–1816. doi: 10.1111/j.1349-7006.2009.01248.x

Figure 1.

Figure 1

c‐Ski inhibits transforming growth factor (TGF)‐β signaling in OCUM‐2MLN cells. (a,b) Overexpression of c‐Ski in OCUM‐2MLN cells. OCUM‐2MLN cells stably expressing hemagglutinin (HA)‐tagged c‐Ski (2MLN‐c‐Ski) were generated by lentivirus‐mediated gene transfer. OCUM‐2MLN cells expressing green fluorescent protein (GFP) (2MLN‐GFP) were used as a control in subsequent experiments. (a) c‐Ski expression in 2MLN‐c‐Ski cells was confirmed by quantitative real‐time RT‐PCR analysis. (b) Cell lysates of parental OCUM‐2MLN cells, 2MLN‐GFP, and 2MLN‐c‐Ski cells were subjected to immunoblotting with anti‐GFP antibody and anti‐HA antibody. GFP and Venus are detected by the anti‐GFP antibody. (c) Inhibition of TGF‐β‐mediated Smad7 and plasminogen activator inhibitor (PAI)‐1 induction by c‐Ski. 2MLN‐GFP and 2MLN‐c‐Ski cells were treated with TGF‐β1 (1 ng/mL) and expression levels of human Smad7 (left) and human PAI‐1 (right) were determined by quantitative real‐time RT‐PCR analysis at the indicated time points.