Figure 3.
Effects of c‐Ski on the growth of OCUM‐2MLN subcutaneous tumors. (a) Growth curves of 2MLN‐green fluorescent protein (GFP) and 2MLN‐c‐Ski tumors in vivo. 2MLN‐GFP or 2MLN‐c‐Ski cells (1 × 107 cells/mouse) were subcutaneously injected into BALB/c nu/nu female mice (6 weeks of age), and subsequent tumor growth was monitored after 1 week. Results are shown as a mean ± SE (*P < 0.05). (b) Gross appearance of 2MLN‐GFP and 2MLN‐c‐Ski tumors. Representative pictures of 2MLN‐GFP and 2MLN‐c‐Ski tumors at day 28 are shown. Note that 2MLN‐c‐Ski tumors exhibit development of neovasculature on the surface. (c) Expression of growth‐regulatory genes in OCUM‐2MLN subcutaneous tumors. Total RNA was extracted from subcutaneous tumors, and the expression levels of p21, c‐myc, and growth arrest and DNA‐damage‐inducible 45β (GADD45β) were examined by quantitative real‐time RT‐PCR analysis. Each value is normalized to the expression of hypoxanthine phosphoribosyltransferase 1 and represents the mean of triplicate determination (*P < 0.05, **P < 0.01, ***P < 0.001).