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Effects of cytokines on the maintenance of the human papillomavirus (HPV) replicon. (A) The HFKp18FLneo was treated with cytokines for 3 days and total DNA was extracted. The DNA was digested with both BglI and DpnI and 2 μg of it was subjected to Southern blot analysis with the DIG‐labeled L1 probe. Normal human foreskin keratinocytes (HFKs) were used as the control (Cr). (B) Expressions of p53 and pRb in the same cells monitored by immunoblot analysis.
