Figure 7.

 The effects of cytokine treatments on human papillomavirus (HPV) replication. Effects of cytokine treatments on HPV replication and on cellular status were examined by using the raft culture. The DNA synthesis of the cells was monitored by the uptake of BrdU. Incorporated BrdU was detected by immunohistochemistry (IHC) with anti‐BrdU antibody. HPV‐DNA was detected by in situ hybridization (ISH) with DIG‐labeled 18L1 probe (ISH). The expressions of p53 and pRb in the raft culture were monitored by IHC with specific antibodies (p53 & pRb).