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. 2010 Apr 23;101(8):1797–1806. doi: 10.1111/j.1349-7006.2010.01598.x

Figure 1.

Figure 1

 Exposure to cisplatin and doxorubicin elevates Twist1 and Y‐box binding protein‐1 (YB‐1) expressions and p300/CBP‐associated factor (PCAF), Twist1, and YB‐1 are up‐regulated in cisplatin‐ and doxorubicin‐resistant cells. (a) KK47 cells were treated with 1 μM of cisplatin for the indicated durations. Quantitative real‐time PCR was performed for PCAF, Twist1, YB‐1, and GAPDH. Each transcript level from untreated KK47 cells was set as 1. Data represent mean ± SD. Nuclear extracts and whole‐cell lysates were subjected to SDS‐PAGE and western blotting analyses were performed with the indicated antibodies. (b) KK47 cells were treated with 10 nM of doxorubicin. Quantitative real‐time PCR and western blotting analyses were performed as described for (a). (c) Quantitative real‐time PCR using cDNA from the indicated cells was performed for PCAF, Twist1, YB‐1, and GAPDH. Each transcript level from KK47 cells was set as 1. Data represent mean ± SD. Whole‐cell lysates were subjected to SDS‐PAGE and western blotting analyses as described for (a). (d) Quantitative real‐time PCR and western blotting analyses were performed as described for (c).