Skip to main content
. 2010 Apr 23;101(8):1797–1806. doi: 10.1111/j.1349-7006.2010.01598.x

Figure 2.

Figure 2

 p300/CBP‐associated factor (PCAF) regulates Y‐box binding protein‐1 (YB‐1) transcription through an interaction with Twist1. (a) Schematic representations of the human YB‐1 gene and various YB‐1 reporter plasmids. (b,c) KK47 (b) and T24 (c) cells (1.5 × 105) were transiently co‐transfected with the indicated amounts of expression plasmids or 20 nM of the indicated siRNA in addition to 0.5 μg of hYB‐1‐Luc and 0.05 μg of pRL‐TK. The luciferase activity of hYB‐1‐Luc alone was set as 1. Data represent mean ± SD. (d) KK47 cells (1.5 × 105) were transiently co‐transfected with 1.0 μg of Myc or Myc‐PCAF expression plasmid in addition to 0.5 μg of the indicated reporter plasmid, and 0.05 μg of pRL‐TK. Luciferase reporter assay was performed as described for (b,c).