Figure 5.
Knockdown of p300/CBP‐associated factor (PCAF) decreases Y‐box binding protein‐1 (YB‐1) expression and retards cancer cell growth in a Twist1‐dependent manner. (a,b) KK47 (a) and T24 (b) cells (3.0 × 105) were transiently transfected with 40 nM of the indicated siRNA. At 72 h after transfection, quantitative real‐time PCR was performed using specific primers and probes for PCAF, Twist1, YB‐1, and GAPDH. Each transcript level from cells transfected with the control siRNA was set as 1. Data represent mean ± SD. Whole‐cell lysates were subjected to SDS‐PAGE, and western blotting analysis was performed using the indicated antibodies. (c) KK47 and T24 cells were transfected with 40 nM of control siRNA, PCAF siRNA #1, or PCAF siRNA #2. At the indicated time points, the cell numbers were counted. The results were normalized by the cell numbers at 0 h. Data represent means ± SD.