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. 2011 Apr 27;102(6):1137–1143. doi: 10.1111/j.1349-7006.2011.01936.x

Figure 1.

Figure 1

  Insig2 mRNA expression levels in cell lines. (a) Insig2 mRNA expression levels in 15 pancreatic cancer cell lines. The expression of Insig2 mRNA was normalized by the expression of 18S rRNA mRNA. All 15 pancreatic cancer cell lines and human pancreatic ductal epithelial (HPDE) cells, as well as two primary cultures of fibroblasts isolated from pancreatic cancer tissues (f2, f3), express Insig2 mRNA. Compared with HPDE cells, SUIT‐2 and KP‐2 cells express higher levels of Insig2 mRNA, whereas the other 13 pancreatic cancer cell lines express lower levels. (b) Comparisons of Insig2 mRNA expression levels under normoxic (21% O2) and hypoxic (1% O2) conditions in two pancreatic cancer cell lines. The Insig2 mRNA expression levels in PANC‐1 and MIA PaCa‐2 cells are 2.3‐fold and 5.2‐fold higher under hypoxic conditions than under normoxic conditions, respectively. (c) Comparisons of the ANGPTL4, ADM, and HIG2 mRNA expression levels under normoxic (21% O2) and hypoxic (1% O2) conditions in the MIA PaCa‐2 cell line. The ANGPTL4, ADM, and HIG2 mRNA expression levels in MIA PaCa‐2 cells are all significantly higher under hypoxic conditions than under normoxic conditions.