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. 2006 Oct 19;98(1):58–67. doi: 10.1111/j.1349-7006.2006.00344.x

Figure 2.

Figure 2

(a) Requirement of direct contact between TKD2 and A549 cells. 1, TKD2 cells alone; 2, TKD2 cells were cultured on the bottom of Boyden chambers, and A549 cells were cultured on the chamber membrane for 24 h; 3, TKD2 cells were cocultured with A549 cells. Matrix metalloprotease (MMP) mRNA levels of TKD2 cells were determined by reverse transcription–polymerase chain reaction (RT‐PCR). (b) Effect of neutralizing antibody against human integrins. Total RNA was extracted from TKD2 cells alone (lane 1), or cocultured with A549 cells (lanes 2–5); 2, without antibody; 3, mouse control IgG (10 µg/mL); 4, antihuman integrin α2 (10 µg/mL); 5, antihuman integrin α4 (10 µg/mL). Antibody was added together with A549 seeded onto the monolayer of TKD2, and incubated for 24 h. MMP mRNA levels of TKD2 cells were determined by real time RT‐PCR. (c) Expression levels of integrin α4 mRNA. Total RNA was prepared from: 1, TIG3; 2, HT1080; 3, A549; 4, HeLaS3; and 5, TMK‐1 cells. Integrin α4 mRNA levels were determined by real time RT‐PCR. (d) Phase‐contrast microscopic observation of A549 cells cocultured on TKD2 cells (1) in the presence of antihuman integrin α4 antibody (1 µg/mL) for 24 h (2).