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. 2006 Oct 19;98(1):58–67. doi: 10.1111/j.1349-7006.2006.00344.x

Figure 9.

Figure 9

(a) Construction of mitochondria‐depleted cells (pseudo ρ0). (a) TKD2 cells were cultured in the presence of ethidium bromide (250 ng/mL) for 1 week (pseudo ρ0). Pseudo ρ0 cells were cultured in the absence of ethidium bromide for 1 week to make revertants (ρ0 rev). Levels of mRNA for mitochondrial markers (complex I, and cytochrome b) were measured by real‐time reverse transcription–polymerase chain reaction (RT‐PCR). (b) Matrix metalloprotease (MMP) mRNA induction in mitochondria‐depleted cells. Control TKD2, pseudo ρ0 or ρ0Rev cells were cocultured with A549 cells, and total RNA was extracted after 24 h. Levels of MMP mRNA were determined by real‐time RT‐PCR.