Figure 9.

Effect of RA‐VII on apoptosis in tumor tissues. Tumor sections obtained from mice on day 15 after inoculation were stained for hematoxylin and eosin (H&E), CD31 and the terminal deoxynucleotidyl transferase‐mediated nick‐end labeling (TUNEL) reaction. CD31 was visualized using biotinylated anti‐CD31 and a streptavidin–Cy3 conjugate, and TUNEL used a 16‐dUTP‐fluorescein conjugate. (A) H&E staining, fluorescent images of CD31 and TUNEL. Scale bars = 200 µm. (B) Quantification of apoptotic cell numbers. Apoptotic cells were counted and calculated as the mean of five fields per section and the values expressed as the mean ± SD of four sections. **P < 0.01 versus vehicle.