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. 2010 Jan 19;101(5):1204–1211. doi: 10.1111/j.1349-7006.2010.01499.x

Figure 6.

Figure 6

 MLN8237 selectively inhibits Aurora A over Aurora B in both human T‐cell leukemia virus type 1 (HTLV‐1)‐ infected and ‐uninfected T‐cell lines. (a) The cells were pretreated for 16 h with 10 nm nocodazole to arrest the cell cycle at mitosis followed by treatment with various concentrations of MLN8237 (0, 12.5, 25, or 50 nm) for 2 h. Western blot analysis was used to detect phospho‐Aurora A (Thr288) and Actin. Similar results were observed in triplicate experiments. (b) Representative immunofluorescent images of the cells treated with 50 nm MLN8237 (MLN [+]) or vehicle (MLN [−]) for 24 h. Cells were stained for anti‐phospho‐histone H3 (Ser10) antibody (p‐histone H3; red) and DNA (DAPI; blue). Arrows indicated mitotic cells. (c) The cells were treated with 12.5 nm MLN8237 (+) or vehicle (−) for 24 h. Phosphrylation of histone H3 (Ser10) and whole histone H3 were analyzed by western blotting. Similar results were observed in triplicate experiments.