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. 2009 Jul 23;100(11):2040–2045. doi: 10.1111/j.1349-7006.2009.01297.x

Figure 4.

Figure 4

 Effect on the level of glucose regulatory protein (GRP58) expression by curcumin, mitomycin (MMC), or their combination with or without SB203580 and PD98059. (A) Relationship between ERK and GRP58. After GRP58 siRNA was transfected, MCF‐7 cells were treated with PD98059 80 μm/L for 48 h. (B) Relation between p38 MAPK and GRP58. After GRP58 siRNA was transfected, the level of GRP58 expression was observed by SB203580 15 μm/L treatment for 48 h. (C) The level of GRP58 expression was regulated by combination treatment via the ERK pathway. The level of GRP58 expression was observed by MMC, curcumin, and their combination treatment with or without PD98059 in MCF‐7 cells. After MCF‐7 cells were pretreated with or without PD98059 80 μm/L for 2 h, cells were treated with MMC 2.5 μm/L, curcumin 40 μm/L, and MMC plus curcumin for 48 h. (D) The level of GRP58 expression was regulated by combination treatment via the p38 MAPK pathway. After MCF‐7 cells were pretreated with or without SB203580 15 μm/L for 2 h, cells were treated with MMC 2.5 μm/L, curcumin 40 μm/L, and MMC plus curcumin for 48 h. Total cellular protein was extracted and western blotting analysis was performed. The density ratio of proteins to GAPDH is shown as relative expression.